Coding

Part:BBa_K2055009:Design

Designed by: Carlos Vasquez   Group: iGEM16_Tec-Monterrey   (2016-09-28)


FUR Protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 122
    Illegal XhoI site found at 307
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 43
    Illegal BsaI.rc site found at 259


Design Notes

You will have to add a FUR Box to repress the transcription with this FUR protein in presence of iron.


Source

The sequence for the FUR Box was obtained from the results of the paper of Quatrini et al. (Citation in our wiki).

References